Epitope mapping

Antibody Technologies provides characterization of antibody binding sites at the amino acid level. While continuous epitopes typically are characterized using traditional peptide library screenings in immunoassays, discontinuous epitopes are characterized by mass spectrometry. Using this approach several antibody binding-sites have been characterized.

Information gathered comprises:

• Relevance of the individual amino acids

• Identification of hot spot amino acids

• Structural requirements

References:

Bergmann et al. Epitope Mapping of Monoclonal Antibodies to Calreticulin Reveals That Charged Amino Acids Are Essential for Antibody Binding. Antibodies (Basel) 2021, 10, 31. doi: 10.3390/antib10030031.

Bergmann et al. Determination of crucial epitopes in the sperm protein calsperin employing synthetic peptides and monoclonal antibodies. J Pept Sci. 2022, e3450. doi: 10.1002/psc.3450.